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Image Search Results
Journal: bioRxiv
Article Title: C-type lectin-like receptor 2 (CLEC-2)-dependent DC migration is controlled by tetraspanin CD37
doi: 10.1101/227918
Figure Lengend Snippet: (A) WT (left), Cd37-/- (middle) or CD11c ΔCLEC”2 (right) DCs were stimulated in a 3D collagen gel with (bottom row) or without (upper row) recombinant podoplanin-Fc (rPDPN-Fc). Cells were stained for F-actin (red) and nucleus (blue) and imaged with a Leica SP5 confocal fluorescence microscope. One representative cell is shown for each condition (overview with more cells is provided in Supplementary Figure 2). Scale bar = 10μm. (B-D) Number (B) and length (C) of actin protrusions, and morphology index (D) of WT (left), Cd37-/- (middle) or CD11c ΔCLEC ” 2 (right) BMDCs upon rPDPN-Fc stimulation (grey boxes) compared to no ligand (white boxes). Data are shown as Tukey Box & whiskers from 3 independent experiments, total n=3 mice per genotype. In Tukey Box & whiskers, black dots are determined as outliers; i.e. data points outside the 25 th and 75 th percentile, minus or plus the 1.5 interquartile range, respectively. Two-way ANOVA with Tukey’s multiple comparisons, ***p<0.001, ****p<0.0001.
Article Snippet: BMDCs (0.3x10 6 ) were seeded into a
Techniques: Recombinant, Staining, Fluorescence, Microscopy
Journal: Scientific Reports
Article Title: Animal-free alternatives for Matrigel in human iPSC-derived blood vessel organoid culture
doi: 10.1038/s41598-025-20091-w
Figure Lengend Snippet: Vascular organoids embedded in fibrin gel show a similar differentiation pattern compared to vascular organoids embedded in Matrigel/collagen. Data shown are from Clones 1 and 2. ( a ) Schematic timeline of the differentiation process for both vascular organoid groups. ( b ) Brightfield images of the vascular organoids during the differentiation process (day 13–18). Scale bar depicts 200 μm. ( c ) Gene expression analysis of vascular organoids at different timepoints. Results represented as mean ± SEM, n = 5 for both groups. One-way ANOVA with Tukey post hoc test. No significant differences between the vascular organoid groups were detected except for VE-Cadherin ( p ≤ 0.05). ( d ) Surface area over time (day 13–18). n = 20 vascular organoids for both groups, 4 technical replicates. Results represented as mean ± SEM. Unpaired T-test between timepoints, no significant differences between the vascular organoid groups were detected. ( e ) Vascular organoid composition based on FACS results. Cell content is represented as the percentage of total cells measured. Results represented as mean ± SEM, n = 5 for both groups. Unpaired T-test, no significant differences between the vascular organoid groups were detected. ( f ) Whole-mount staining of vascular organoids. Vascular organoids are stained for endothelial cell marker CD31 (Red) and mural cell marker CD140b (Green). Scale bar depicts 50 μm.
Article Snippet:
Techniques: Clone Assay, Gene Expression, Staining, Marker